Journal: Nature

Article Title: Structure of the Insulin Receptor-Insulin Complex by Single Particle CryoEM analysis

doi: 10.1038/nature26153

Figure Lengend Snippet: Human IR ECD binding and kinetic data . Top: Human IR ECD binding data for recombinant human insulin. Data is reported as the geometric mean for 23 replicates; the geometric standard deviation is shown in parentheses. The potency measured with the 125 I-insulin competition assay agrees very well with values from the literature 17 . Bottom: Human IR ECD Binding Kinetic Data for RHI. Data is reported as the geometric mean for three replicates; the geometric standard deviation is shown in parentheses. The SPR data is very consistent with values from the literature 31 .

Article Snippet: Recombinant human Insulin Receptor/CD220 was obtained from R&D systems (catalog # 1544-IR-50/CF, https://www.rndsystems.com/products/recombinant-human-insulin-r-cd220-aa-28-944-protein-cf_1544-ir-cf ), reconstituted in PBS (Phosphate buffered saline, pH=7.4) at 0.3 mg/ml (0.00293 mM), stored in 100 ul aliquots at -80, and used as is (without further purification).

Techniques: Binding Assay, Recombinant, Standard Deviation, Competitive Binding Assay